Postmitotic Expression of SOD1^G93A Gene Affects the Identity of Myogenic Cells and Inhibits Myoblasts Differentiation

Figure 7

The acquisition of fibro-adipogenic features involves a HDAC-regulated network. (a) Upper panel shows western blot analysis of HDAC4 expression in C2C12 and C2C12 MLC/ cells and densitometric analysis (lower panel) of the ratio between HDAC4 and α-tubulin. (b) Real time PCR for BAF60C in C2C12 (white bars) and C2C12 MLC/ (black bars). (c, d) Histograms of the percentage of Sca1 (c) and positive α7 integrin cells (d) of control C2C12 MLC/ (black bars) cells and TSA treated C2C12 MLC/ cells (grey bars). D2 referred to days of culture in differentiation condition. (e) Representative immunofluorescence analysis for MHC after 5 days in differentiation medium in untreated (left panel) and TSA treated (right panel) C2C12 MLC/ cells. (f) Histograms of the differentiation index (left panel) and fusion index (right panel) in control (black bars) and treated cells (grey bars). D2 and D5 referred to days of culture in differentiation condition. Data are represented as mean ± SEM (; ).

(a)

(b)

(c)

(d)

(e)

(f)