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Mediators of Inflammation
Volume 2015 (2015), Article ID 543541, 11 pages
Research Article

Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma

1Analytical BioSciences, LACDR, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands
2Sino-Dutch Centre for Preventive and Personalized Medicine, Leiden University, P.O. Box 9502, 2300 RA Leiden, Netherlands
3SU Biomedicine, Utrechtseweg 48, 3700 AJ Zeist, Netherlands
4Discovery Sciences, Janssen Research & Development, Turnhoutseweg 30, 2340 Beerse, Belgium
5TNO Innovation for Life, P.O. Box 360, 3700 AJ Zeist, Netherlands
6Tohoku Institute of Technology, Sendai 982-8577, Japan

Received 28 August 2015; Accepted 8 October 2015

Academic Editor: Nina Ivanovska

Copyright © 2015 Min He et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group. The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analyses were applied. The results in univariate Student’s t-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-κB because of insufficient PPAR-γ ligands.