Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma

Table 1

List of oxylipins detected in mice plasma, measured by multiple reaction monitoring (precursor ions → product ions) in LC-MS/MS analysis.

Compounds	MS transitions (/)	value	VIP	Regulation	Pathway
LA
9,10-DiHOME	313.2 → 201.1	0.0002	1.86	↓	CYP450
12,13-DiHOME	313.2 → 183.2	0.006	1.51	↓	CYP450
9,10-EpOME	295.2 → 171.2	0.028	1.27	↓	CYP450
12,13-EpOME	295.2 → 195.2	0.096	1.00	↓	CYP450
9-KODE	293.2 → 185.2	0.003	1.61	↓	5-LOX
9,12,13-TriHOME	329.2 → 211.2	0.017	1.36	↓	5-LOX
9,10,13-TriHOME	329.2 → 171.1	0.026	1.29	↓	5-LOX
9-HODE	295.2 → 171.1	0.052	1.14	↓	5-LOX
13-KODE	293.2 → 113.1	0.082	1.04	↓	12/15-LOX
13-HODE	295.2 → 195.2	0.733	0.21	—	12/15-LOX
EPA
12-HEPE	317.2 → 179.1	0.016	1.37	↑	12/15-LOX
DHA
14-HdoHE	343.2 → 205.0	0.010	1.45	↑	ROS
13-HdoHE	343.2 → 281.0	0.012	1.42	↑	ROS
10-HdoHE	343.2 → 153.0	0.035	1.23	↑	ROS
17-HdoHE	343.2 → 281.3	0.173	0.83	—	12/15 LOX
19,20-DiHDPA	361.2 → 273.3	0.509	0.41	—	CYP450
DGLA
6-keto-PGF1α	369.2→ 163.1	0.390	0.53	—	COX
8-HETrE	321.3 → 303.0	0.469	0.45	—	12/15 LOX
AA
8-HETE	319.2 → 155.1	0.074	1.06	↑	12/15-LOX
12-HETE	319.2 → 179.2	0.116	0.95	↑	12/15 LOX
15-HETE	319.2 → 219.2	0.770	0.18	—	12/15-LOX
5-HETE	319.2 → 115.1	0.713	0.23	—	5-LOX
13,14-dihydro-PGF2α	355.2 → 275.3	0.112	0.96	↑	COX
PGF2α	353.2 → 193.1	0.176	0.82	—	COX
13,14-dihydro-15-keto-PGF2α	353.2→ 183.1	0.618	0.31	—	COX
12S-HHTrE	279.2 → 179.2	0.733	0.21	—	COX
TXB2	369.2 → 169.1	0.900	0.08	—	COX
14,15-DiHETrE	337.2 → 207.2	0.662	0.27	—	CYP450
9-HETE	319.2 → 167.1	0.408	0.51	—	ROS
11-HETE	319.2 → 167.1	0.820	0.14247	—	ROS

The oxylipins are grouped based on the original polyunsaturated fatty acid precursor: linoleic acid (LA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), dihomo-γ-linolenic acid (DGLA), and arachidonic acid (AA). Their metabolic pathways include enzymatic pathways: cyclooxygenase (COX), lipoxygenase (LOX), cytochrome P450 (P450), and nonenzymatic reactive oxygen species (ROS) pathway. The significance of changes between two groups was illustrated by value from univariate test (Student’s -test) and VIP score from multivariate test (PLS-DA). The important regulations in the CIA group were marked with “↓” or “↑” selected based on VIP scores.
↓: downregulated in CIA group.
↑: upregulated in CIA group.