Chemerin15-Ameliorated Cardiac Ischemia-Reperfusion Injury Is Associated with the Induction of Alternatively Activated Macrophages

Figure 2

C15 reduced cardiomyocyte apoptosis and neutrophil infiltration. (a) Representative photographs of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling- (TUNEL-) stained ischemic area 24 h after reperfusion. For these images, apoptotic nuclei are indicated by TUNEL staining (green), and total nuclei are indicated by 4,6-diamidino-2-phenylindole (DAPI) staining (blue); bar, 10 μm. (b) Quantitative analysis of TUNEL-positive nuclei over the total number of nuclei in vivo (). (c) Caspase-3 activity in the ischemic myocardium was assessed after 24 h of reperfusion (). (d) Malonaldehyde+4-hydroxy-alkenals (MDA+4HAE) contents in the ischemic myocardium were evaluated using a lipid peroxidation assay (). (e) Cardiac myeloperoxidase (MPO) activity was detected in ischemic myocardia (). < 0.05 versus the sham group; < 0.05 versus the I/R group.

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