Research Article
The Impact of ATRA on Shaping Human Myeloid Cell Responses to Epithelial Cell-Derived Stimuli and on T-Lymphocyte Polarization
Table 2
Expression of CD1a, DC-SIGN, and CD14 cell surface molecules on monocyte-derived cells generated in the presence of the colony stimulating factors GM-CSF+IL-4, GM-CSF, or M-CSF. Peripheral blood-derived monocytes were differentiated in the presence of GM-CSF+IL-4, GM-CSF, or M-CSF in RPMI + 10% FCS for 3 days and the expression of the cell surface markers CD1a, DC-SIGN/CD209, and CD14 was monitored by flow cytometry using fluorescent labeled specific antibodies. The expression levels of CD1a, DC-SIGN, and CD14 are indicated as mean fluorescence intensity (MFI).
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