Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) Downregulates the Expression of Protumor Factors Cyclooxygenase-2 and Inducible Nitric Oxide Synthase in a GM-CSF Receptor-Independent Manner in Cervical Cancer Cells

Figure 3

Overexpression of GM-CSF downregulated COX-2 and iNOS expression in cervical cancer cell lines. (a, b) Western blot analysis of Ca Ski, SiHa, and Hela cells untransfected or transfected with pGM-CSF or pcDNA3.1 to detect the expression of GM-CSF (a) and iNOS and COX-2 (b), respectively. (c) Unpaired Student’s -test was used to detect the differences of iNOS and COX-2 expression in different groups. Each experiment was repeated three times. GT: pGM-CSF transfected cells, VT: vector pcDNA3.1 transfected cells (vector control), and NT: nontreated cells (blank control). , versus NT group. Error bars represent mean ± SD.

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