Multidrug Resistance Protein-4 Influences Aspirin Toxicity in Human Cell Line
Aspirin stimulates endogenous MRP4 mRNA and protein expression in Hek-293 cells. (a) Q-RT-PCR analysis of endogenous MRP4 α expression after 48 h of treatment with 50 μM aspirin. Data were normalized with β-actin expression and reported as mean ± SD of increasing fold, compared to control (). (b) Representative western blot, of 3 experiments performed, of endogenous MRP4 expression after 48 h treatment with 50 μM aspirin. Aspirin untreated cells were used as control cells. (c) Densitometric analysis of western blot is reported as mean ± SD of increasing fold, compared to control ().