Figure 2: Dynamics of circulating free (cf) DNA and quantification of host-derived and bacteria-derived cf-DNA. (a) Amount of cf-DNA in the plasma at 6 and 24 hours after cecal ligation and puncture (CLP) or sham operation ( mice per group). (b) Amount of bacteria-derived DNA, quantified based on 16s rDNA by quantitative real-time polymerase chain reaction (PCR) ( mice per group). (c) Amount of host-derived DNA, quantified based on mouse beta-2-microgloblin by quantitative real-time PCR ( mice per group).