miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8

Figure 3

IL-8 is a direct target of miR-106a. (a) Up: potential sites in IL-8 3′UTR targeted by miR-106a. 3′UTR of IL-8 was cloned into a luciferase reporter vector. Mutated sequences were generated in the seed regions to abolish binding of the corresponding miRNAs. Down: HEK293 cells were cotransfected with miR-106a mimics or inhibitor and the luciferase reporter constructs harboring IL-8 or mutant IL-8 3′UTR fragments. The luciferase reporter assays were performed 48 h after transfection. The luciferase activities were measured and normalized to a Renilla luciferase activity. (b) miR-106a expression level was determined after transfection with miR-106a mimics or inhibitor. (c) IL-8 mRNA and protein levels after transfection with miR-106a mimics were assessed by qRT-PCR and ELISA. (d) IL-8 mRNA and protein levels after transfection with miR-106a inhibitor were assessed by qRT-PCR and ELISA. .

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