Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes

Figure 1

Amarogentin inhibits substance P- (SP-) triggered mediator release from the human mast cell line LAD-2. LAD-2 cells were stimulated with increasing concentrations of SP. LAD-2 cells stimulated with 2 μM SP were preincubated with amarogentin (100 μM) or the positive control azelastine (24 μM). A part of the amarogentin stimulated group was preincubated for 30 min with the PLC inhibitor U73122 (10 μM). Histamine (a) and TNF-α (b) release was measured 20 min after SP stimulation. The data are shown with standard deviation (SEM) and were analyzed with the unpaired, two-tailed -test (; ns: not statistically significant; ).

(a)

(b)