Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes

Figure 3

Amarogentin does not inhibit IL-8 and MMP-1 secretion in stimulated HaCaT keratinocytes. HaCaT cells were costimulated with 10 μM histamine and 25 ng/mL TNF-α and preincubated with amarogentin (100 μM) or azelastine (24 μM) for 30 minutes. A part of the amarogentin stimulated group was preincubated for 30 min with the PLC inhibitor U73122 (10 μM). After 24 h IL-8 (a) and MMP-1 (b) expression was assessed in the cell supernatant. The data are shown with standard deviation (SEM) and were analyzed with the unpaired, two-tailed -test (; bs: borderline significant , ns: not statistically significant; ).

(a)

(b)