Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes

Figure 4

Amarogentin inhibits NGF secretion in stimulated HaCaT keratinocytes. HaCaT cells were costimulated with 10 μM histamine and 25 ng/mL TNF-α and preincubated with amarogentin (100 μM) or the control azelastine (24 μM) for 30 minutes. After 4 h NGF RNA expression was assessed. The data are shown with standard deviation (SEM) and were analyzed with the unpaired, two-tailed -test (ns: not statistically significant; ).