IL-1 Receptor Blockade Alleviates Graft-versus-Host Disease through Downregulation of an Interleukin-1-Dependent Glycolytic Pathway in Th17 Cells

Figure 2

Treatment with IL-1Ra inhibits Th17 cell development via downregulation of the glycolysis pathway. Splenic CD4⁺T cells from C57BL6 mice were cultured under Th17-polarizing conditions in the presence or absence of IL-1Ra for 3 days. (a) The proportion of Th17 cells or cells was determined using flow cytometric analysis. (b) The supernatants were collected, and an enzyme-linked immunosorbent assay (ELISA) was performed to quantify the production of TGF-β and IL-17. ((c) and (d)) The mRNA levels of IL-17 and glycolysis-related factors and enzymes were measured using real-time PCR. , , and . Data are representative of 2 independent experiments.

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