Hypoxic Preconditioning Suppresses Glial Activation and Neuroinflammation in Neonatal Brain Insults

Figure 4

Hypoxia insult induces glial activation in cultured glial cells without reducing cell viability. Nitrite production and cell viability were determined in a primary mixed glial culture (a) and in the BV-2 microglial cell line (b) after exposure to hypoxia, at various time intervals (as indicated). Hypoxia durations up to 6 h increased nitrite production in a time-dependent manner without reducing cell viability. Hypoxia exposure for 8 h was able to induce a low level of cell death. The data represent the mean ± standard error of the mean (SEM), based on five independent experiments in each group. ((a), (b)) compared with HI group.

(a)  

(b)