Subtoxic Concentrations of Hepatotoxic Drugs Lead to Kupffer Cell Activation in a Human In Vitro Liver Model: An Approach to Study DILI

Figure 4

Induction of oxidative stress in PHH. PHH from three individual donors (A, B, and C, see Table 1) were treated with 100 μM acetaminophen (APAP) or 100 μM diclofenac (DIC) for 1 h. Intracellular ROI formation ((a1), (b1), and (c1)) was investigated by the DCF assay and the cell activity ((a2), (b2), and (c2)) was determined by the XTT assay. Data are shown as box plots, representing the median, the interquartile range, and minimum and maximum values, .