Genetic Deletion of Soluble Epoxide Hydrolase Attenuates Inflammation and Fibrosis in Experimental Obstructive Nephropathy

Figure 5

Knockout of sEH decreases the UUO-induced collagen deposition and expression of fibrosis-related proteins. ((a) and (b)) 14 days after UUO surgery, kidneys were harvested. Kidney sections from WT and sEH^−/− mice were subjected to Masson’s trichrome staining or Sirius Red Staining. Scale bar: 100 μm. (c) Kidney lysates were immunoblotted with collagen 1A1 (COL1A1), α-actin, and GAPDH. Data are mean ± SEM from 8 mice. versus WT nonobstructed kidney; versus WT UUO kidney.

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