Figure 7: Deletion of sEH attenuates UUO-elicited increases in ROS production and NADPH oxidase activity. ((a) and (b)) Three days after UUO surgery, kidneys were harvested from WT and mice and subjected to measurement of and H2O2 by use of HE/ethidium and DCFH-DA/DCF assays. (c) NADPH oxidase activity of kidney lysates was analyzed by NADP+/NADPH assay kit. Data are mean ± SEM from 8 mice. versus WT nonobstructed kidney; versus WT UUO kidney.