Genetic Deletion of Soluble Epoxide Hydrolase Attenuates Inflammation and Fibrosis in Experimental Obstructive Nephropathy

Figure 7

Deletion of sEH attenuates UUO-elicited increases in ROS production and NADPH oxidase activity. ((a) and (b)) Three days after UUO surgery, kidneys were harvested from WT and mice and subjected to measurement of and H₂O₂ by use of HE/ethidium and DCFH-DA/DCF assays. (c) NADPH oxidase activity of kidney lysates was analyzed by NADP+/NADPH assay kit. Data are mean ± SEM from 8 mice. versus WT nonobstructed kidney; versus WT UUO kidney.

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