EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection

Figure 4

EPSTI1 exhibits anti-HCV activity. (a) Huh7.5 cells were transfected with p3XFLAG-CMV-EPSTI1 or vector followed by HCV infection (MOI = 0.1). The cells were incubated with IL-28A (5 ng/mL), IFN-α (0.5 U/mL) for 48 h, or without treatment as a control. Intracellular HCV RNA was detected with real-time PCR and normalized with GAPDH. (b) EPSTI1 shRNA or control shRNA was delivered into Huh7.5 cells. The cells were infected with HCV (MOI = 0.1) and incubated with IL-28A (20 ng/mL), IFN-α (5U/mL) for 48 h, or without treatment as a control. (c) The cells were treated as described in part (b). The proteins of NS5A and EPSTI1 were detected with western bolt. (d) Huh7.5 cells were transfected with p3XFLAG-CMV-EPSTI1/pSilencer-EPSTI1 or vectors followed by HCV infection (MOI = 0.1). The intracellular infectious virus and the secreted infectious virus were detected with FFU assay. HCV RNA in the supernatant was extracted and detected with real-time PCR.

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