EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection

Figure 7

EPSTI1 upregulates PKR expression by activating PKR promoter. (a) HLCZ01 cells were transfected with p3XFLAG-CMV-EPSTI1 or/and pSilencer-IFNAR1 followed by HCV infection (MOI = 0.1). PKR mRNA was detected by real-time PCR and normalized with GAPDH. (b) Huh7.5 cells were transfected with p3XFLAG-CMV-EPSTI1 followed by HCV infection. The cells were assayed by immunofluorescence at 48 h postinfection. ((c) and (d)) Huh7.5 (c) and HLCZ01 cells (d) were cotransfected with pGL3-PKR promoter and p3XFLAG-CMV-EPSTI1 or pSilencer-EPSTI1 followed by HCV infection. Cell extracts were made at 24 h postinfection followed by luciferase determination.

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