Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway

Figure 5

The effect of M. oleifera flower extract on NF-κB p65 expression in LPS-stimulated RAW 264.7 macrophages. Macrophages were treated with extract (100 and 200 µg/mL) and dexamethasone (0.5 μg/mL) in the presence of LPS (1 µg/mL) for 24 hours. Expression of NF-κB p65 was observed by fluorescence microscope after immunofluorescence staining with anti-NF-κB p65 antibody and fluorescein labeled anti-rabbit IgG (red). Nuclei of the cells were stained with Hoechst 33342 (blue) and images were captured (original magnification, ×200).