Moringa oleifera Flower Extract Suppresses the Activation of Inflammatory Mediators in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages via NF-κB Pathway

Figure 6

(a) Anti-inflammatory effect of 80% hydroethanolic M. oleifera bioactive flower extract on the expression of NF-κB, IκB-α, iNOS, and COX-2 in LPS-induced RAW 264.7 macrophages. A density of 1 × 10⁶ cells/well of macrophages in the presence or absence of LPS were seeded in 6-well plate and treated with indicated concentrations of flower extract for 24 h. The protein of cells was collected through RIPA buffer and analysed by Western blotting. β-actin acts as a loading control and also standard for target proteins in quantitative determination. (b) Densitometry analysis results of the effect of M. oleifera flower extract on proteins expression. and were LPS-treated group versus control; : treated group significantly different from LPS-treated group. Control: basal level of cytokines released without LPS induction. The data are presented as mean ± SD of three independent experiments.

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