ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury

Figure 3

NLRP3 inflammasome inhibitor BAY11-7082 attenuated burn-induced NLRP3 activation in lungs. Rats were subjected to burn injury. BAY11-7082 was administered intraperitoneally immediately after burn injury in rats. At 24 h and 48 h after burn injury, lung tissue was collected. The mRNA expressions ((a) and (b)) and protein level change ((c), (d), and (e)) of NLRP3 and Caspase-1 were analyzed by real-time PCR and immunoblots in lung tissue after burn with BAY11-7082 treatment. The NLRP3 positive signal was determined by immunohistochemistry (f) and was quantified (g) in (f) per mm² in lung tissue after burn with BAY11-7082 treatment. The values presented are mean ± SEM ( per group). .

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