ROS-Mediated NLRP3 Inflammasome Activity Is Essential for Burn-Induced Acute Lung Injury

Figure 5

BAY11-7082 inhibited the NLRP3 inflammasome signals of AMs challenged with burn serum. AMs cells were exposed to 10% sham serum (0 h in burn group), 10% sham serum + BAY11-7082 (0 h in burn + BAY group), 10% burn serum, and 10% burn serum + BAY11-7082 for 24 h or 48 h. BAY11-7082 was treated immediately after serum challenge. Real-time PCR showed the mRNA expression ((a) and (b)) of NLRP3 and Caspase-1 in macrophages exposed to burn serum for 24 h or 48 h. Immunoblots showed the protein level change ((c), (d), and (e)) of NLRP3 and Caspase-1 in each group. The release of IL-1β (f) and IL-18 (g) in the supernatant was tested by ELISA. Results represent mean ± SEM of six independent experiments. . AM, alveolar macrophage.

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