Interleukin-1β Affects MDAMB231 Breast Cancer Cell Migration under Hypoxia: Role of HIF-1α and NFκB Transcription Factors

Figure 1

IL-1β increases MDAMB231 cell migratory ability under hypoxia. Data are shown as means ± SEM. , statistically significant difference between IL-1β-treated hypoxic cells (IL-1β) and hypoxic control cells (Ctr). (a) The migration assay was performed as described in Section 2. The migration index was calculated as a fold change of relative CyQuant fluorescence of cells migrating into the lower chamber normalized to that of the untreated samples (Ctr) (). Migrating cells were photographed and photomicrographs of representative fields from the indicated conditions were shown. (b) Protein expression of HIF-1α was determined by Western blot analysis at indicated time points. β-actin was used as loading control. A representative blot from three independent experiments is shown. Quantification of HIF-1α protein expression was achieved by chemiluminescence. (c) CXCR1 mRNA expression was determined by qRT-PCR analysis (). (d) Protein expression of phospho-p38 was determined by Western blot analysis. (e) VEGF-A mRNA and protein expression were analyzed by qRT-PCR and ELISA (). (f) Protein and mRNA expression of NFκB was determined by Western blot and qRT-PCR analysis (). (g) CXCL8 mRNA and protein expression were analyzed by qRT-PCR and ELISA ().

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