Interleukin-1β Affects MDAMB231 Breast Cancer Cell Migration under Hypoxia: Role of HIF-1α and NFκB Transcription Factors

Figure 2

HIF-1α siRNA does not affect IL-1β-induced cell migration in hypoxia. Data are shown as means ± SEM. , statistically significant difference between IL-1β-treated hypoxic cells silenced for HIF-1α (HIF-1α siRNA) and IL-1β-treated hypoxic cells silenced with oligo controls (Ctr siRNA). (a) HIF-1α mRNA expression was determined by qRT-PCR analysis (). (b) Protein expression of HIF-1α was determined by Western blot analysis. β-actin was used as loading control. A representative blot from three independent experiments was shown. Quantification of HIF-1α protein expression was achieved by chemiluminescence. (c) CXCR1 mRNA expression was determined by qRT-PCR analysis (). (d) The migration index was calculated as a fold change of relative CyQuant fluorescence of cells migrating into the lower chamber normalized to that of the control samples (Ctr siRNA) (). (e and f) CXCL8 and NFκB mRNA expression was analyzed by qRT-PCR ().

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