Interleukin-1β Affects MDAMB231 Breast Cancer Cell Migration under Hypoxia: Role of HIF-1α and NFκB Transcription Factors

Figure 3

Topotecan does not reduce the induction of hypoxic breast cancer cell migration by IL-1β. Data are shown as means ± SEM. , statistically significant difference between IL-1β-treated hypoxic cells in the presence of Topotecan (IL-1β + TPT) versus IL-1β-treated hypoxic cells in the absence of Topotecan (IL-1β). (a) Protein expression of HIF-1α was determined by Western blot analysis. A representative blot from three independent experiments is shown. HIF-1α protein quantification was achieved by chemiluminescence. (b) Cell migration index was calculated as a fold change of relative fluorescence of cells migrating into the lower chamber normalized to that of the untreated samples (IL-1β) (). (c and d) CXCL8 and NFκB mRNA expression was analyzed by qRT-PCR ().

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