Interleukin-1β Affects MDAMB231 Breast Cancer Cell Migration under Hypoxia: Role of HIF-1α and NFκB Transcription Factors

Figure 4

Role of NFκB in IL-1β-induced hypoxic cell migration. Data are shown as means ± SEM. , statistically significant difference between IL-1β-treated hypoxic cells in the presence of BAY 11-7082 (IL-1β + BAY) and IL-1β-treated hypoxic cells in the absence of BAY 11-7082 (IL-1β). (a) Protein expression of NFκB was determined by Western blot analysis and a representative blot from three independent experiments is shown. Quantification of NFκB protein expression was achieved by chemiluminescence (). (b) The migration index was calculated as a fold change of relative fluorescence of cells migrating into the lower chamber normalized to that of the untreated samples (IL-1β) (). (c) CXCL8 mRNA and protein expression were analyzed by qRT-PCR and ELISA ().

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