Captopril Pretreatment Produces an Additive Cardioprotection to Isoflurane Preconditioning in Attenuating Myocardial Ischemia Reperfusion Injury in Rabbits and in Humans

Figure 5

Isoflurane (Iso) and captopril (Cap) attenuated postischemic myocardial apoptosis in rabbits. (a) Protein expression of cardiac Bcl-2, (b) protein expression of cardiac Bax, (c) ratio of Bcl-2 to Bax, (d) representative graphs showing the proportion of apoptotic myocytes, and (e) quantitation of apoptotic myocytes. Sham: sham operated control; I/R: rabbits subjected to myocardial ischemia reperfusion (MI/R); Cap: rabbits treated with captopril (25 mg/kg given intravenously 24 h) prior to inducing MI/R; Iso: rabbits received isoflurane preconditioning (15 min 1.1% end tidal isoflurane followed by a 15 min washout period before inducing MI/R); Iso+Cap: rabbits received captopril in combination with Iso. Data are shown as means ± SD, with animals per group. versus I/R, versus IPC.

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