Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis
Table 2
Lymphocyte populations (%).
Sham
Saline
A100
A200
A400
Blood
T cells
17.4 ± 0.8
13.1 ± 1.3
12.9 ± 0.8
13.0 ± 1.0
11.7 ± 0.8
Th cells
58.4 ± 1.0
51.4 ± 1.6
51.8 ± 1.5
53.4 ± 1.4
53.7 ± 1.4
Tc cells
32.9 ± 2.8
33.7 ± 3.3
36.0 ± 3.7
36.8 ± 2.8
35.1 ± 3.1
B cells
49.8 ± 1.6
31.1 ± 1.4
30.2 ± 2.6
25.6 ± 2.7
22.3 ± 3.0
Spleen
T cells
24.2 ± 0.6
20.4 ± 1.0
19.7 ± 0.6
18.9 ± 0.7
19.6 ± 0.7
Th cells
47.6 ± 1.3
39.9 ± 1.2
42.5 ± 0.8
43.5 ± 1.0
44.1 ± 0.8
Tc cells
34.4 ± 0.7
36.1 ± 0.7
34.0 ± 0.6
33.3 ± 0.3
34.0 ± 1.1
B cells
47.1 ± 1.4
48.8 ± 1.4
48.9 ± 1.4
50.5 ± 1.4
49.1 ± 1.3
Peyer’s patches
T cells
8.4 ± 0.5
11.7 ± 1.0
15.6 ± 2.4
16.2 ± 1.8
17.4 ± 2.4
Th cells
53.8 ± 3.7
28.4 ± 1.3
27.3 ± 3.2
31.7 ± 4.1
29.1 ± 2.5
Tc cells
16.9 ± 2.0
14.4 ± 1.7
15.0 ± 2.8
16.6 ± 2.5
15.3 ± 2.5
B cells
77.8 ± 1.9
58.1 ± 2.4
52.7 ± 5.6
52.9 ± 5.2
53.6 ± 6.1
CD45-positive cells were gated to determine the population of lymphocytes by flow cytometry. Populations of T and B cells were determined as the percentages of CD3ε+CD19− and CD3ε−CD19+ cells among CD45-positive cells. Percentages of T helper (Th) and cytotoxic T (Tc) cells were, respectively, determined by CD4+CD8− and CD4−CD8+ cells in CD3ε-expressing T lymphocytes. Values are presented as the mean ± SEM. Significantly different from the sham group (). A100, A200, and A400, respectively, indicate treatment with 100, 200, and 400 mg Astragalus polysaccharides/kg of body weight.
