Modulatory Effects of Astragalus Polysaccharides on T-Cell Polarization in Mice with Polymicrobial Sepsis

Table 4

Expression of T-cell polarization-related genes in the spleen.

	Sham	Saline	A100	A200	A400
Relative mRNA ratio
IFN-γ	1.07 ± 0.06	0.33 ± 0.03	0.42 ± 0.10	0.46 ± 0.03	0.42 ± 0.08
IL-4	1.06 ± 0.04	2.52 ± 0.20	1.55 ± 0.20^†	1.52 ± 0.17^†	1.13 ± 0.26^†
IL-17A	1.06 ± 0.06	0.43 ± 0.07	0.71 ± 0.07	0.72 ± 0.12	0.90 ± 0.17^†
Foxp3	1.00 ± 0.07	0.78 ± 0.07	0.53 ± 0.06	0.54 ± 0.02	0.55 ± 0.11
IL-6	1.00 ± 0.02	0.45 ± 0.03	0.92 ± 0.12	0.96 ± 0.12	1.33 ± 0.23^†
IL-2	1.02 ± 0.10	0.53 ± 0.08	0.57 ± 0.10	0.65 ± 0.05	0.64 ± 0.08
TGF-β	1.00 ± 0.09	0.79 ± 0.07	0.92 ± 0.09	0.83 ± 0.04	0.98 ± 0.08

The relative amount of mRNA was calculated using the comparative Ct method, and mRNA expression of sham-operated mice was used as a calibrator. Data are presented as the mean ± SEM. Significantly different from the sham group (). ^†Significantly different from the saline group ().
A100, A200, and A400, respectively, indicate treatment with 100, 200, and 400 mg Astragalus polysaccharides/kg of body weight.
IFN: interferon; IL: interleukin; TGF: transforming growth factor.