The Effect of Therapeutic Blockades of Dust Particles-Induced Ca²⁺ Signaling and Proinflammatory Cytokine IL-8 in Human Bronchial Epithelial Cells

Figure 1

Size distribution of PM₁₀ dust particles and PM₁₀-induced Ca²⁺ signaling and ROS signal in human bronchial epithelial BEAS-2B cells. (a) Diameter of PM₁₀ dust particles. Three kinds of shape represent independent applications to the particle analyzer. (b) Changes in induced by 50 μg/mL PM₁₀ in 1 mM Ca²⁺ medium and (c) in Ca²⁺-free medium. Top bars on trace panels indicate the extracellular solutions with which the cells were treated. (d) Number of responding cells in different dose of PM₁₀ at 0.05, 0.075, 10, 50, and 100 μg/mL PM₁₀. (e) Time-dependent ROS signal after exposure to 50 μg/mL PM₁₀. The fluorescence of H2DCFDA was subsequently visualized by confocal laser scanning microscopy and quantified, and the relative intensities were calculated by setting the fluorescence intensity of control cells at 0 min to a value of 1. Data are represented as mean ± SE. values were considered significant.

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