The Effect of Therapeutic Blockades of Dust Particles-Induced Ca²⁺ Signaling and Proinflammatory Cytokine IL-8 in Human Bronchial Epithelial Cells

Figure 2

The additive effect of PM₁₀-induced Ca²⁺ signal in store-operated Ca²⁺ influx machinery. The 50 μM CPA-induced Ca²⁺ increase in the presence of 2 mM extracellular Ca²⁺ in the (a) absence or (b) presence of 50 μg/mL PM₁₀. The top bars on trace panels indicate the type of extracellular solutions applied to the cells and traces are the reading from an experimental average. (c) Analysis of CPA-induced Ca²⁺ release (α), influx (), and sustained Ca²⁺ (γ) was determined using fluorescence ratios from baseline (dotted). Results are means ± SE from three independent experiments. values were considered significant. (d) 100 μM Lanthanum chloride (La³⁺), a nonselective cation inhibitor. Trace is the reading from an experimental average. (e) PM₁₀-induced Ca²⁺ increase in the presence of 10 μM nifedipine, an inhibitor of voltage dependent L-type Ca²⁺ channels. Each trace is the reading from a single cell.

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