The Effect of Therapeutic Blockades of Dust Particles-Induced Ca²⁺ Signaling and Proinflammatory Cytokine IL-8 in Human Bronchial Epithelial Cells

Figure 3

PM₁₀-induced Ca²⁺ signaling is associated with the PLC/IP₃ pathway. (a) 50 μg/mL PM₁₀-induced Ca²⁺ signal in the presence of PLC inhibitor 5 μM U73122 or its inactive analog 5 μM U73343. (b) The 50 μg/mL PM₁₀-induced Ca²⁺ signal was prevented by 20 mM caffeine, IP₃R antagonist. (c) The 50 μg/mL PM₁₀-induced Ca²⁺ signal was completely abolished by 10 μM BAPTA, AM. The top bars on trace panels indicate the type of extracellular solutions applied to the cells. (d) Data are represented as mean ± SE. values were considered significant. All traces are the reading from an experimental average. The dotted line shows the baseline at ratio 1.

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