The Effect of Therapeutic Blockades of Dust Particles-Induced Ca²⁺ Signaling and Proinflammatory Cytokine IL-8 in Human Bronchial Epithelial Cells

Figure 6

The modulation of PM₁₀-induced IL-8 mRNA expression by several Ca²⁺ blockades. (a) PM₁₀ treatment increased IL-8 mRNA expression. Cells were stimulated with 50 μg/mL PM₁₀ for the indicated time (). The total mRNA of stimulated cells was extracted and quantified after the value was normalized to GAPDH. (b–d) IL-8 mRNA expression treated with 50 μg/mL PM₁₀ for 90 min in the pretreatment of all compounds for 30 min, as used in Ca²⁺ measurement experiments (). The total mRNA of stimulated cells was extracted and quantified after the value was normalized to GAPDH and represented the ratio of IL-8/GAPDH (fold change to that of vehicle). Data are represented as mean ± SE. values were considered significant.

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