Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis

Figure 2

Blockade of CysLTR₁ induces a less severe pulmonary infection in susceptible but not in resistant mice. 1 × 10⁶ P. brasiliensis yeasts were inoculated into A/J and B10.A mice treated or not with montelukast (MTL) during 3 days (10 mg/kg/day by the oral route) starting 24 h before infection. The severity of infection was assessed 48 h later by determining fungal loads (CFU) in the lungs (a). The severity of infection was also assessed in B10.A mice treated or not with MTL and infected with 1 × 10⁵ and 1 × 10⁴ P. brasiliensis yeasts (b). The levels of NO in the lung homogenates of MTL-treated and untreated B10.A mice infected with 1 × 10⁶ and 1 × 10⁵ P. brasiliensis yeasts were measured by Griess reagent. The dashed line indicates the levels +/− 2 SD of NO levels measured in the lungs of untreated, noninfected mice (c). Data are means ± SEM of three experiments, .

(a)

(b)

(c)