Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis

Figure 3

Blocking of CysLTR₁ alters the expression of ALOX5, ALOX15, NOS2, and ARG1 mRNA. Quantitative PCR analysis of ALOX5, ALOX15, NOS2, and ARG1 genes expression. A/J and B10.A mice were untreated or treated with montelukast (MTL) during 3 days (10 mg/kg/day by the oral route) starting 24 h before infection and infected with 1 × 10⁶ P. brasiliensis yeasts. Total RNA from whole lungs was obtained 48 h after infection, reverse transcribed, and cDNA amplified. Real-time PCR was performed using TaqMan universal master mix. Amplified products were normalized to the amount of GAPDH products. Data represent the means ± SEM of at least 5 mice/group and were obtained in two independent experiments. ( and ).