Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis

Figure 5

Inhibition of CysLT signaling does not alter the chronic phase of P. brasiliensis infection, but 5-LO deficiency results in less severe disease. MTL was administered by oral route in the dose of 10 mg/kg from day −1 until day 10 (a) or from day −1 until day 56 (b) after infection with 1 × 10⁶ yeast cells, and B10.A mice were sacrificed at week 8 after infection. The severity of infection was evaluated by determining fungal loads (CFU assays) in the lungs, livers, and spleens. The effect of LTs production was also studied using mice genetically deficient in the enzyme 5-LO. The recovery of fungal loads (CFU) from organs of 5-LO^−/− and control WT129Svj mice was measured 8 weeks after infection (c). The bars represent mean ± SEM log₁₀ numbers of CFU obtained from groups of 5-6 mice. The results were obtained in two independent experiments. .

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(b)

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