Research Article
Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis
Figure 8
Exogenous lipoxin reduces, and blockade of lipoxin receptor increases the fungal loads of macrophages. IFN-γ primed (20 ng/mL) and unprimed macrophages from B10.A mice were treated with several doses of synthetic lipoxin (10−9, 10−7, and 10−6 M) or a specific antagonist of lipoxin receptor (BOcMLP) (10−5, 10−6, and 10−4 M) and infected with P. brasiliensis in a macrophage-yeast cells rate of 50 : 1. After 48 h of cocultivation, the monolayers were lysed and assayed for the presence of viable yeasts by a CFU assay. Data are means ± SEM of results from quintuplicate samples from three independent experiments. .