HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation

<div>TNF-α induced NF-κB translocation into the nucleus. Using an anti-p65 primary antibody, NF-κB was detected in HT-29-NF-κB-hrGFP E5 cells. Cells were stimulated with TNF-α (50 ng/mL) during 1 h, nonstimulated cells were used as control. Nuclei were stained with Hoescht 33342 (blue). Nonstimulated cells show only NF-κB (red) in their cytoplasm (a); meanwhile, in stimulated cells NF-κB is present both in the cytoplasm and nucleus (b). Scale bar = 10 μm.</div>

Mediators of Inflammation

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Figure 6

Figure 6: HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation 