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Mediators of Inflammation
Volume 2015, Article ID 860534, 13 pages
Research Article

HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation

Cell Biology Unit, Institut Pasteur de Montevideo, Mataojo 2020, 11400 Montevideo, Uruguay

Received 6 November 2014; Revised 25 February 2015; Accepted 26 February 2015

Academic Editor: Flavio Caprioli

Copyright © 2015 Giuliana Mastropietro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In absence of primary antibody anti-p65, no signal was detected in HT-29-NF-κB-hr-GFP E5 clone cells incubated with secondary antibody anti-rabbit Alexa 594. Immunofluorescence was performed as previously described in Materials & Methods.

  1. Supplementary Material