HT-29 and Caco-2 Reporter Cell Lines for Functional Studies of Nuclear Factor Kappa B Activation

Table 1

Determination of EC50, detection limit, and linear range for different clones.

	Caco-2-NF-B-hrGFP		HT-29-NF-B-hrGFP
	Clone C3		Clone E5		Clone F6
	TNF-	IL-1	TNF-	IL-1	TNF-	LPS
EC50 (ng/mL)	ND	0.23 (0.16–0.33)	0.52 (0.47–0.59)	0.21 (0.15–0.28)	0.72 (0.43–1.22)	0.15 (0.10–0.21)
Detection limit (ng/mL)	0.050	0.022	0.012	0.014	0.053	0.018
Linear range (ng/mL)	0.050–1,000	0.008–1.000	0.050–3.125	0.008–0.200	0.050–3.125	0.040–0.800

Different clones were seeded, cultured ON, and treated with TNF-α, LPS, or IL-1β. Cells were trypsinized and GFP expression was analyzed by flow cytometry. Data was normalized and EC50, detection limit, and linear range were calculated using nonlinear regression 3-parameter fit using GraphPad Prism. 95% confidence intervals are shown between brackets. ND: not determined.