Bone Components Downregulate Expression of Toll-Like Receptor 4 on the Surface of Human Monocytic U937 Cells: A Cell Model for Postfracture Immune Dysfunction

Figure 2

Regulation of TLR4 expression by bone component (BC) exposure in U937 cells not mediated by transcriptional and posttranscriptional modifications. (a) Actinomycin D chase experiment to evaluate the stability of TLR4 mRNA in 50 ng/mL BC-treated U937 cells. (b) U937 cells were treated with 0.5–50 ng/mL BC for the indicated time (4 or 24 h). TLR4 mRNA levels were analyzed by quantitative real-time PCR after normalization to GAPDH mRNA. Data are presented as mean ± SEM ().

(a)

(b)