The Combination of N-Acetyl Cysteine, Alpha-Lipoic Acid, and Bromelain Shows High Anti-Inflammatory Properties in Novel In Vivo and In Vitro Models of Endometriosis

Figure 3

Characterization of the purity of endothelial cells isolated from endometriotic tissue. (A) EECs were characterized by cytofluorimetric analysis for the expression of CD45, CD34, CD31, CD105, vWF, and vimentin, and the expression of these markers (green lines) was compared with correlated control antibodies (black lines). The expression of vWF and CD31 was confirmed by immunofluorescence, on EECs grown to confluence in 8-chamber culture slides. After fixation and permeabilization the cells were stained with mAb anti-human CD31 or anti-vWF. The binding of mouse monoclonal antibodies was revealed by the incubation with goat anti-mouse IgG FITC-conjugated secondary antibodies. Nuclei were stained in blue by DAPI: original magnification 200x.