Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

Figure 2

GAL is not able to induce an inflammatory response. (a) Schematic representation of the model used for this study. The cultures of human macrophages were treated with 10 ng/mL LPS or 10 μg/mL GAL for 24 h. The controls were not treated. Supernatants were harvested and TNFα (b), IL6 (c), and IL-10 (d) proteins levels were evaluated by CBA (); , compared with LPS. (e) The culture of human macrophages was treated with GAL at various concentrations for 24 h. Supernatants were harvested and the TNFα protein levels were evaluated by CBA (). (f) The culture of human macrophages was treated with GAL at various concentrations for 24 h. Cells were harvested, and intracellular cells stained with 7AAD were analyzed by flow cytometry. The 7AAD negative cells are represented (percentage of cell viability, ).

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