Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

Figure 3

The NFκB2/p100 accumulation after the first LPS or GAL challenge. Cultures of human macrophages were challenged with 10 ng/mL LPS or 10 μg/mL GAL for 24 h. The controls were not treated. The levels of NFκB2/p100 or IRAK-M and β-actin were studied by Western blot analysis of the cytosolic fraction. (a) A NFκB2/p100 standard blot is shown (). (b) Densitometry analysis: arbitrary units [AU] of NFκB2/p100 bands are normalized with respect to -actin (). , compared with LPS. (c) A IRAK-M standard blot is shown (). (d) Densitometry analysis: arbitrary units [AU] of IRAK-M bands are normalized with respect to -actin ().

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