Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

Figure 4

GAL is mediated by dectin-1 receptor. (a) Schematic representation of the inhibitor receptor of GAL model used for this study. The cultures of human macrophages were pretreated with or without WGPs (dectin-1 receptor inhibitor) 200 μg/mL for 1 hour. Next, GAL was added to the cells for 24 h, and then cells were washed three times and cultured in resting for 16 h. Cells were challenged with 10 ng/mL LPS for 5 h or 24 h. The controls were not pretreated. Supernatants were harvested at 5 h for TNFα (b), 24 h for IL6 (c), and 5 h for IL10 (d). Proteins levels were evaluated by CBA (); compared with LPS. Levels of NFκB2/p100 and -actin were studied by Western blot analysis of the cytosolic fraction. (e) A NFκB2/p100 standard blot is shown (). (f) Densitometry analysis: arbitrary units [AU] of NFκB2/p100 bands are normalized with respect to -actin (). compared with LPS.

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