Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

Figure 5

The attenuated inflammation effect induced by GAL is reverted by small interfering RNA of NFκB2/p100. Cultures of siRp100 and sirRcontrol transfected human monocyte/macrophages were treated with 10 g/mL GAL or 10 ng/mL LPS for 24 h. The controls were not treated. The levels of NFκB2/p100 and β-actin were studied by Western blot analysis of the cytosolic fraction. (a) A NFκB2/p100 and β-actin standard blot are shown (). (b) TNF protein level was evaluated by CBA (); , compared with siRcontrol.

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