Synergistic Stimulation with Different TLR7 Ligands Modulates Gene Expression Patterns in the Human Plasmacytoid Dendritic Cell Line CAL-1

Figure 3

Analysis of regulatory networks triggered by 9.2s RNA plus CL264. CAL-1 cells were seeded into 24-well plates. After overnight resting, they were stimulated with CL264 (5 µg/mL) and 9.2s RNA (2 µg/mL). After incubation for 4 hrs, cells were lysed and total RNA was extracted. Following cDNA synthesis, labeled cDNA was hybridized to microarrays. The network analysis identifies major (red) and minor regulatory nodes (yellow) of the synergistic regulation.