Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes

Figure 1

Hypoxia and OLN effects on human monocyte viability. Human adherent monocytes (10⁶ cells/2 mL Panserin 601 medium) were left untreated or treated with increasing doses (100–400 μL) of OLNs or with 200 μL OFNs or OSS for 24 h in normoxia (20% O₂, black columns/squared-lines) or hypoxia (1% O₂, white columns/squared-lines). After collection of cell supernatants and lysates, cytotoxicity percentage was measured through LDH assay (panels (a) and (c)), whereas cell viability percentage was measured through MTT assay (panels (b) and (d)). Results are shown as means + SEM from three independent experiments. Data were also evaluated for significance by ANOVA. No significant differences between normoxic and hypoxic control cells or between OLN-treated and untreated cells were observed (all panels).

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