Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes

Figure 4

Hypoxia and OLN effects on protein levels of gelatinase inhibitors (TIMP-1 and TIMP-2) secreted by human monocytes. Human adherent monocytes (10⁶ cells/2 mL Panserin 601 medium) were left untreated or treated with 200 μL OLNs, OFNs, or OSS for 24 h in normoxia (20% O₂; black columns, both panels) or hypoxia (1% O₂; white columns, both panels). After collection of cell supernatants, TIMP-1 (panel (a)) and TIMP-2 (panel (b)), protein levels were quantified by ELISA. Results are shown as means + SEM from three independent experiments. Data were also evaluated for significance by ANOVA: versus normoxic control cells: (panel (a)) and not significant (panel (b)); versus hypoxic control cells: (panel (a)) and not significant (panel (b)).

(a)

(b)